proto script ii reverse transcription kit (New England Biolabs)
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New England Biolabs
proto script ii reverse transcription kit
Proto Script Ii Reverse Transcription Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proto+script+ii+reverse+transcription+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/us12612395-1489-17-23
Average 99 stars, based on 1837 article reviews
Proto Script Ii Reverse Transcription Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proto+script+ii+reverse+transcription+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/us12612395-1489-17-23
Average 99 stars, based on 1837 article reviews
proto script ii reverse transcription kit - by Bioz Stars,
2026-09
99/100 stars
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Synthesized:Article Title: Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer. Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma. Article Snippet: For siRNA knockdown, 6 × 105 A673 cells, at 60% confluency and cultured in Opti-MEM ReducedSerum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nMusing Lipofectamine RNAiMAXTransfection Reagent: GLI1-1 (J003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-140005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNAwas isolated fromcells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Rocaglate derivatives and uses thereof Article Snippet: For shRNA knockdown, 2×106 GBM0308 cells per well were seeded in 6-well plates and transduced with 750 μL lentivirus particles in a total volume of 1 mL of NBE medium supplemented with 8 μg/mL polybrene (Sigma, Cat #1003). .. Quantitative RT-PCR: Total RNA was extracted from cells using Trizol (Invitrogen, Cat #15596). cDNA was synthesized using Article Title: Identification of Rocaglate Acyl Sulfamides as Selective Inhibitors of Glioblastoma Stem Cells Article Snippet: .. Quantitative RT-PCR Total RNA was extracted from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Inhibiting the GPI Transamidase Subunit GPAA1 Abolishes CD24 Surface Localization and Enhances Macrophage-Mediated Phagocytosis of Ovarian Cancer Cells Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma Article Snippet: For siRNA knockdown, 6 × 10 5 A673 cells, at 60% confluency and cultured in Opti-MEM Reduced-Serum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nM using Lipofectamine RNAiMAX Transfection Reagent: GLI1-1 (J-003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-14-0005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO-2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNA was isolated from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Reverse Transcription:Article Title: Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer. Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma. Article Snippet: For siRNA knockdown, 6 × 105 A673 cells, at 60% confluency and cultured in Opti-MEM ReducedSerum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nMusing Lipofectamine RNAiMAXTransfection Reagent: GLI1-1 (J003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-140005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNAwas isolated fromcells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Rocaglate derivatives and uses thereof Article Snippet: For shRNA knockdown, 2×106 GBM0308 cells per well were seeded in 6-well plates and transduced with 750 μL lentivirus particles in a total volume of 1 mL of NBE medium supplemented with 8 μg/mL polybrene (Sigma, Cat #1003). .. Quantitative RT-PCR: Total RNA was extracted from cells using Trizol (Invitrogen, Cat #15596). cDNA was synthesized using Article Title: Identification of Rocaglate Acyl Sulfamides as Selective Inhibitors of Glioblastoma Stem Cells Article Snippet: .. Quantitative RT-PCR Total RNA was extracted from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Inhibiting the GPI Transamidase Subunit GPAA1 Abolishes CD24 Surface Localization and Enhances Macrophage-Mediated Phagocytosis of Ovarian Cancer Cells Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma Article Snippet: For siRNA knockdown, 6 × 10 5 A673 cells, at 60% confluency and cultured in Opti-MEM Reduced-Serum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nM using Lipofectamine RNAiMAX Transfection Reagent: GLI1-1 (J-003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-14-0005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO-2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNA was isolated from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Real-time Polymerase Chain Reaction:Article Title: Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer. Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma. Article Snippet: For siRNA knockdown, 6 × 105 A673 cells, at 60% confluency and cultured in Opti-MEM ReducedSerum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nMusing Lipofectamine RNAiMAXTransfection Reagent: GLI1-1 (J003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-140005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNAwas isolated fromcells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Rocaglate derivatives and uses thereof Article Snippet: For shRNA knockdown, 2×106 GBM0308 cells per well were seeded in 6-well plates and transduced with 750 μL lentivirus particles in a total volume of 1 mL of NBE medium supplemented with 8 μg/mL polybrene (Sigma, Cat #1003). .. Quantitative RT-PCR: Total RNA was extracted from cells using Trizol (Invitrogen, Cat #15596). cDNA was synthesized using Article Title: Identification of Rocaglate Acyl Sulfamides as Selective Inhibitors of Glioblastoma Stem Cells Article Snippet: .. Quantitative RT-PCR Total RNA was extracted from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: Inhibiting the GPI Transamidase Subunit GPAA1 Abolishes CD24 Surface Localization and Enhances Macrophage-Mediated Phagocytosis of Ovarian Cancer Cells Article Snippet: .. The cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma Article Snippet: For siRNA knockdown, 6 × 10 5 A673 cells, at 60% confluency and cultured in Opti-MEM Reduced-Serum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nM using Lipofectamine RNAiMAX Transfection Reagent: GLI1-1 (J-003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-14-0005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO-2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNA was isolated from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Isolation:Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma. Article Snippet: For siRNA knockdown, 6 × 105 A673 cells, at 60% confluency and cultured in Opti-MEM ReducedSerum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nMusing Lipofectamine RNAiMAXTransfection Reagent: GLI1-1 (J003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-140005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNAwas isolated fromcells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma Article Snippet: For siRNA knockdown, 6 × 10 5 A673 cells, at 60% confluency and cultured in Opti-MEM Reduced-Serum Medium (Gibco Cat# 31985-070), were transfected with the following siRNAs (obtained from Dharmacon (Colorado, USA)) at 100 nM using Lipofectamine RNAiMAX Transfection Reagent: GLI1-1 (J-003896-05-0005), GLI1-2 (L-003896-00-0005), GLI2-1 (A-006468-14-0005), GLI2-2 (A-006468-15-0005), SMO-1 (A-005726-13-0005), SMO-2 (L-005726-00-0005), or a control pool (D-001810-10-05). .. Total RNA was isolated from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using Quantitative RT-PCR:Article Title: Rocaglate derivatives and uses thereof Article Snippet: For shRNA knockdown, 2×106 GBM0308 cells per well were seeded in 6-well plates and transduced with 750 μL lentivirus particles in a total volume of 1 mL of NBE medium supplemented with 8 μg/mL polybrene (Sigma, Cat #1003). .. Quantitative RT-PCR: Total RNA was extracted from cells using Trizol (Invitrogen, Cat #15596). cDNA was synthesized using Article Title: Identification of Rocaglate Acyl Sulfamides as Selective Inhibitors of Glioblastoma Stem Cells Article Snippet: .. Quantitative RT-PCR Total RNA was extracted from cells using Trizol (Invitrogen, Cat# 15596). cDNA was synthesized using |